Astrocytes

We have a number of plasmids and viruses that we would be glad to share!
Many of these are also available on Addgene or the UPenn Vector Core and UNC vector cores.
For more information, please contact Dr. Kathryn Reissner, kreissne@email.unc.edu

Plasmids

  • GfaABC1D-GLT1-His (see Li et al (2014) JNeurosci 34(22):7622 and Logan et al (2018) Neuropharmacology 135:297-307)
  • GfaABC1D-GCaMP6s.WPRE.SV40
  • GfaABC1D-cytoGCaMP6f.WPRE.SV40 (note: Addgene 52925 does not have a WPRE)
  • GfaABC1D-LckGCaMP6f.WPRE.SV40 (note: Addgene 52924 does not have a WPRE)

Viruses

  • GfaABC1D-GLT1-His in AAV5 and AAV8 (see Li et al (2014) JNeurosci 34(22):7622 and Logan et al (2018) Neuropharmacology 135:297-307.)
  • GfaABC1D-GCaMP6s.WPRE.SV40 in AAV5
  • GfaABC1D-LckGFP in AAV5 (see Scofield et al (2016) Biol Psychiatry 80(3):207-15 and Shigetomi et al (2013) J Gen Physiol 141(5):633-47.)
  • GfaABC1D-cytoGCaMP6f (Addgene 52925) in AAV5
  • GfaABC1D-LckGCaMP6f (Addgene 52924) in AAV5